STUDIES ON INHIBITORS OF VIRUS DISEASES INFECTING SOME LEGUMINOUS CROPS IN EGYPT

Awad, M. A.; Ghar, A. I. Abo El-; Ahmed, A. A.; Attar, A. K. A. El; Awad, H. M. A.

doi:10.21608/mjapam.2017.126189

STUDIES ON INHIBITORS OF VIRUS DISEASES INFECTING SOME LEGUMINOUS CROPS IN EGYPT

Document Type : Original Article

Authors

¹ Prof. of Plant Pathology, Fac. of Agric., Menoufia Univ.

² Head Researcher of virus and phytoplasma.Plant Pathology. Res. Inst., ARC

³ Senior Researcher of virus and phytoplasma .Plant Pathology. Res. Inst., ARC

⁴ Lecturer of Plant Patholog Faculty of Agric., Menoufia Univ.

10.21608/mjapam.2017.126189

Abstract

The Objectives of this study were to:

-   Isolate and identify Bean yellow mosaic virus affecting bean and faba bean plants under field conditions from different locations in Menoufia governorate. Yellowing, mosaic malformations and blisters were appeared on infected leaves. Symptomatic plants were tested by biological methods as host range and indicator plants, serological tests and PCR method.
-   Study of some inhibitors (Plant extracts and salicylic acid (SA) against BYMV. Evaluate systemic acquired resistance (SAR) using host local lesions (Chenopodium amaranticolor) and Dianthus caryophyllus extract was found to be the most effective inducer (95.8%).
-   Study the biochemical changes in induced, infected and healthy plants, photosynthesis pigments (chlorophyll a, chlorophyll b and carotenoids), oxidative enzymes (peroxidase and polyphenol oxidase) and antiviral protein.
-   Examine the histological changes using light and electron microscopy, revealed changes as affecting on mesophyll layers, spongy tissue and decrease in number of chloroplasts and increase of starch grains in infected plants compared with healthy and induced plants.
-   Also, Evaluate the SAR under field conditions, our results were based on determination of chlorophyll pigments, oxidative enzymes and the yield (the dry weight of pods and seeds) for treatments healthy and infected plants.

Keywords